An Evaluation of the Treatment of Full-Thickness Wounds Using Adipose Micro-Fragments within a Liquid Dermal Scaffold

In full-thickness wounds, inflammation, lack of matrix deposition, and paucity progenitor cells delay healing. As commercially available solid (sheet) scaffolds are unable to conform wounds varying shapes sizes, we previously generated a nutritious, injectable, liquid skin substitute that can wound topography. combination with adipose micro-fragments as viable source cells, composite, in situ forming was tested for the treatment silicon ring splinted rats. The vitro survivability migratory capacity adipocytes derived from rat micro-fragmented fat cultured our scaffold examined Live/Dead assay, showing viability migration after 7 14 days. vivo, efficacy alone (LDS) or (LDS+A) compared standard dressing protocol (NT). LDS LDS+A showed ameliorated healing, including complete epithelialization less immune cell infiltration, NT control. Our findings demonstrate 3D is rich environment adipocyte migration, addition this be used treating wounds.